Andrea Puhar

Research qualifications: Docent

Contact

andrea.puhar@umu.se

+46 90 786 65 42

Works as

Visiting associate professor at Department of Molecular Biology Section: Group Andrea Puhar

6K och 6L, Sjukhusområdet Umeå universitet, 901 87 Umeå

Antibacterial compounds against non-growing and intracellular bacteria

npj Antimicrobials and Resistance, Springer Nature 2025, Vol. 3, (1)

Kaldalu, Niilo; Bērziņš, Normunds; Berglund Fick, Stina; et al.

Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei

Nature Communications, Springer Nature 2025, Vol. 16, (1)

Miles, Sydney L.; Santillo, Dilys; Painter, Hannah; et al.

Extracellular ATP is an environmental cue in bacteria

Cell Reports, Elsevier 2025, Vol. 44, (10)

Tronnet, Sophie; Pandey, Vikash; Lloret-Berrocal, Miriam; et al.

CRISPR-cas-guided mutagenesis of chromosome and virulence plasmid in Shigella flexneri by cytosine base editing

mSystems, American Society for Microbiology 2023, Vol. 8, (1)

Sharma, Atin; Omer Aden, Ruqiya; Puhar, Andrea; et al.

At-home sampling to meet geographical challenges for serological assessment of SARS-CoV-2 exposure in a rural region of northern Sweden, March to May 2021: a retrospective cohort study

Eurosurveillance, European Centre for Disease Control and Prevention (ECDC) 2023, Vol. 28, (13)

Wigren, Julia; Vikström, Linnea; Rosendal, Ebba; et al.

RpuS/R is a novel two-component signal transduction system that regulates the expression of the pyruvate symporter MctP in Sinorhizobium fredii NGR234

Frontiers in Microbiology, Frontiers Media S.A. 2022, Vol. 13

Ramos, Ana Laura; Aquino, Maria; García, Gema; et al.

Infection-induced membrane ruffling initiates danger and immune signaling via the mechanosensor PIEZO1

Cell Reports, Elsevier 2022, Vol. 40, (6)

Tadala, Lalitha; Langenbach, Dorothee; Dannborg, Mirjam; et al.

Vibrio cholerae cytotoxin MakA induces noncanonical autophagy resulting in the spatial inhibition of canonical autophagy

Journal of Cell Science, The Company of Biologists 2021, Vol. 134, (5)

Corkery, Dale; Nadeem, Aftab; Aung, Kyaw Min; et al.

Generation of plasma cells and CD27^-IgD^- B cells during hantavirus infection is associated with distinct pathological findings

Clinical & Translational Immunology (CTI), John Wiley & Sons 2021, Vol. 10

Kerkman, Priscilla; Dernstedt, Andy; Tadala, Lalitha; et al.

Whole-Genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria

Bio-protocol, Bio-protocol 2020, Vol. 10, (18)

Cervantes-Rivera, Ramón; Puhar, Andrea

Complete genome sequence and annotation of the laboratory reference strain Shigella flexneri serotype 5a M90T and genome-wide transcriptional start site determination

BMC Genomics, Springer Nature 2020, Vol. 21, (1)

Cervantes-Rivera, Ramón; Tronnet, Sophie; Puhar, Andrea

Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells

Bio-protocol, Bio-Protocol 2019, Vol. 9, (13)

Sharma, Atin; Puhar, Andrea

Gentamicin protection assay to determine the number of intracellular bacteria during infection of human TC7 intestinal epithelial cells by Shigella flexneri

Bio-protocol, Bio-Protocol 2019, Vol. 9, (13)

Sharma, Atin; Puhar, Andrea

PI5P Triggers ICAM-1 Degradation in Shigella Infected Cells, Thus Dampening Immune Cell Recruitment

Cell Reports, Cell Press 2016, Vol. 14, (4) : 750-759

Boal, Frédéric; Puhar, Andrea; Xuereb, Jean-Marie; et al.

Distinct mutations led to inactivation of type 1 fimbriae expression in Shigella spp

PLOS ONE, Vol. 10, (3)

Bravo, Verónica; Puhar, Andrea; Sansonetti, Philippe; et al.

Type III secretion system

Current Biology, Vol. 24, (17) : R784-R791

Puhar, Andrea; Sansonetti, Philippe J

Induction of Connexin-hemichannel Opening

Bio-protocol, Vol. 4, (17)

Puhar, Andrea; Sansonetti, Philippe J.

Dye-uptake Experiment through Connexin Hemichannels

Bio-protocol, Vol. 4, (17)

Puhar, Andrea; Sansonetti, Philippe J.

A Shigella effector dampens inflammation by regulating epithelial release of danger signal ATP through production of the lipid mediator PtdIns5P

Immunity, Vol. 39, (6) : 1121-1131

Puhar, Andrea; Tronchère, Hélène; Payrastre, Bernard; et al.

Preventing acute gut wall damage in infectious diarrhoeas with glycosylated dendrimers

EMBO Molecular Medicine, Vol. 4, (9) : 866-881

Teo, Ian; Toms, Steve M.; Marteyn, Benoit; et al.

The Shigella flexneri type three secretion system effector IpgD inhibits T cell migration by manipulating host phosphoinositide metabolism

Cell Host and Microbe, Vol. 9, (4) : 263-272

Konradt, Christoph; Frigimelica, Elisabetta; Nothelfer, Katharina; et al.

Haemolysin II is a Bacillus cereus virulence factor that induces apoptosis of macrophages

Cellular Microbiology, Vol. 13, (1) : 92-108

Tran, Seav-Ly; Guillemet, Elisabeth; Ngo-Camus, Maud; et al.

Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus

PLOS ONE, Vol. 6, (9)

Tran, Seav-Ly; Puhar, Andrea; Ngo-Camus, Maud; et al.

cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin

Biochemical and Biophysical Research Communications - BBRC, Vol. 376, (2) : 429-433

Dal Molin, Federica; Zornetta, Irene; Puhar, Andrea; et al.

NADH oxidation drives respiratory Na+ transport in mitochondria from Yarrowia lipolytica

Archives of Microbiology, Vol. 190, (4) : 471-480

Lin, Po-Chi; Puhar, Andrea; Steuber, Julia

Anthrax edema toxin modulates PKA- and CREB-dependent signaling in two phases

PLOS ONE, Vol. 3, (10)

Puhar, Andrea; Dal Molin, Federica; Horvath, Stéphanie; et al.

The concerted action of the Helicobacter pylori cytotoxin VacA and of the v-ATPase proton pump induces swelling of isolated endosomes

Cellular Microbiology, Vol. 9, (6) : 1481-1490

Genisset, Christophe; Puhar, Andrea; Calore, Federica; et al.

Where and how do anthrax toxins exit endosomes to intoxicate host cells?

Trends in Microbiology, Vol. 15, (11) : 477-482

Puhar, Andrea; Montecucco, Cesare

A vertebrate-type ferredoxin domain in the Na+-translocating NADH dehydrogenase from Vibrio cholerae

Journal of Biological Chemistry, Vol. 280, (24) : 22560-22563

Lin, Po-Chi; Puhar, Andrea; Türk, Karin; et al.

Comparison of the pH-induced conformational change of different clostridial neurotoxins

Biochemical and Biophysical Research Communications - BBRC, Vol. 319, (1) : 66-71

Puhar, Andrea; Johnson, E A; Rossetto, O; et al.

NADH oxidation by the Na+-translocating NADH: quinone oxidoreductase from Vibrio cholerae

Journal of Biological Chemistry, Vol. 279, (20) : 21349-21355

Türk, Karin; Puhar, Andrea; Neese, Frank; et al.

PIEZO1 activation during invasive infection triggers broad changes in the transcriptional landscape

Tadala, Lalitha; Cervantes-Rivera, Ramón; Puhar, Andrea

View publications in DiVA

Research groups

Group Leader, MIMS

Andrea Puhar lab

Research projects

1 January 2022 until 31 December 2024

Mechanism and inflammatory impact of extracellular ATP signaling in intestinal bacteria

Published: 10 Nov, 2022

50 million SEK to new research within UCMR

Published: 10 Aug, 2022

A new way to detect infection